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Human-CD14+-Monocytes

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Human-CD14+-Monocytes

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	Print Version
Description	Human CD14+ Monocytes are immature phagocytic cells circulating in the blood that phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into macrophages as well as dendritic cells in vitro, both of which are important in host defense. These cells are useful in immunology-related research including phagocytic function and signaling, cellular immunity and antigen presentation studies. Human CD14+ Monocytes can mature into 1) M1- or M2- macrophages using abm’s M1-Macrophage Differentiation Medium (TM006) or M2-Macrophage Differentiation Medium (TM007), respectively, or 2) dendritic cells using abm’s Dendritic Cell Differentiation Medium (TM008).
Procedure Overview
Biosesafety Level	II
Organism	Homo sapiens
Source Organ	Blood
Isolation	These cells are extracted from whole blood using Lymphoprep(R), a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.
Growth Properties	slightly adherent
Passage Number	They are cryopreserved immediately after isolation
Population Doubling	The Human CD14+ Monocytes do not proliferate in vitro
Markers	CD14+
Applications	For Research Use Only. The most common applications of CD14+ monocytes are their in vitro differentiation into monocyte-derived dendritic cells and macrophages, respectively.
Disease	Normal
Propagation	The base medium for this cell line is Prigrow II medium available in abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: heat-inactivate fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The Human CD14+ Monocytes do not proliferate in vitro
Quality Control	abm’s cryopreserved PBMCs consistently exhibited viability above 87% (Day 0 when thawed) with less than a 7.5% deviation from that of fresh PBMCs.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.