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Details

Host: Rabbit

Isotype: IgG

Class: Polyclonal

Immunogen: Synthetic peptide corresponding to HA

Applications:

Red-colored affinity matrix consists of purified anti-HA rabbit polyclonal antibody (Clone HA-7) covalently attached to agarose beads. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA). Convenient for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-HA Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cells.

Technical Specifications:

• Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%) 
• Concentration: 25 mg Red Separopore ® per mL of buffer
• Particle size range: 52 – 165 µm 
• Ligand coupling method: CNBr activation 
• Binding capacity: ~400 µg HA-tagged fusion protein/mL drained gel
• Exclusion limit: approximately 2 x 10⁷ 
• pH stability: 3-9 (for use), 2-10 (for cleaning) 
• Supplied as 50% suspension in PBS, containing 50% glycerol. 

Additional Information

Additional Information