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Details

Host: Rabbit

Isotype: IgG

Class: Polyclonal

Immunogen: Synthetic peptide corresponding to Human c-Myc protein

Applications:

Red-colored affinity matrix consists of affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to red beaded agarose (Separopore®). The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. Anti-cMyc Red-Separopore® 4B-CL is convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-cMyc Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.

Red Separopore ® Technical Specifications:

• Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
• Concentration: 25 mg Red Separopore ® per mL of buffer
• Particle size range: 52 – 165 µm
• Ligand coupling method: CNBr activation 
• Binding capacity: approximately 50 µg/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning) 
• Supplied as 50% suspension in PBS, containing 50% glycerol. 

Additional Information

Additional Information