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PMSF

品牌：AG Scientific

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规格：5 g

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CAS:329-98-6Formula:C7H7FO2SMW:174.2Appearance:WhiteCrystallineSolidPurity:98%

商品详情参考文献相关资料

An irreversible inhibitor of serine proteases (such as thrombin, trypsin and chymotrypsin) and mammalian acetylcholinesterase. It causes sulfonylation of the active-site serine residues. Inhibits cysteine proteases like papain (reversible by DTT treatment). Also inhibits internucleosomal DNA fragmentation in immature thymocytes.

PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature. Working Concentration: 0.1-1.0mM.

Additional Information

Synonyms	Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride
Product #	P-2401
CAS #	329-98-6
Chemical Name	α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride
Formula	C₇H₇FO₂S
MW	174.2
Appearance	White Crystalline Solid
Purity	98%
Solubility	Soluble in 10% pure ethanol, chloroform and diethyl ether. Clear, colorless solution in chloroform and diethyl ether.
Melting Point	93.8°C
Preparation	Dissolve PMSF in isopropanol at 1.74 mg/mL (10 mM); store in aliquots at -20°C.
Storage Temp	Store desiccated at room temperature. Protect from moisture.
Use	PMSF is a serine protease inhibitor commonly used in the preparation of cell lysates