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Applications: Tosyl-activated 4B-CL is used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups to the matrix. Tosyl-activated 4B-CL couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interfering with the antigen binding region.

Technical specifications:

• Matrix active group: p-toluenesulfonyl chloride (tosyl chloride)
• Matrix: 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Groups coupled to: Primary amines at pH range 9 – 10
• Phosphate, HEPES, bicarbonate and borate buffers should be used
• Binding capacity: 4 – 8 mg chymotrypsin /ml matrix

Additional Information

Additional Information