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Fluorogenic substrate for caspase-3, with K=9.7M. C1127 issimilar to Ac-DEVD-AMC (# C-1133) but the AFC fluorophore has a greater Stokes shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. Ex.:400nm, Em.: 505nm.

Additional Information

Synonyms	Ac-Asp-Glu-Val-Asp-AFC, Caspase-3 Substrate (AFC), Ac-DEVD-AFC, Caspase -1, Caspase -4, Caspase -7
Product #	C-1127
Chemical Name	Ac-Asp-Glu-Val-Asp-AFC
Formula	C30H34N5O13F3
MW	729.6
Appearance	Off-White Powder
Purity	95%
Solubility	Soluble in DMF, DMSO or Methanol.
Storage Temp	-20°C

Additional Information

MDL Number	MFCD01310970
ChemACX	X1206324-4
InChI	InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
SMILES	CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C
Peptide Sequence	AC-ASP-GLU-VAL-ASP-7-AFC