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Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins. Guanidine Hydrochloride is also been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step. Guanidine Hydrochloride is an inhibitor of RNase.

Additional Information

Product #	G-2589
CAS #	50-01-1
Formula	CH5N3•HCl
MW	95.53
Appearance	Crystalline powder
Purity	≥99%
Solubility	Soluble in water (6 M) at 20° C, ethanol (300 g/l), and alcohol.
Melting Point	185-189° C
Storage Temp	Store at room temperature

Additional Information

SMILES	C(=N)(N)N.Cl