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Applications:

bioWorld`s Endotoxin Affisorbent™consists of immobilized polymyxin B on Separopore® 4B-CL. The polymyxins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymyxin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide. The immobilized polymixin B gel is a stable affinity matrix that resists leaching of ligand into the valuable preparation. Affisorbent™ Endotoxin Removing Gel can also be used to remove endotoxins from nucleic acid (DNA) samples.Polymyxin B-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B- Separopore® 4B-CL has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes. Polymyxin B- Separopore® 4B-CL is also used for the removal of endotoxin levels in protein solutions, cell culture media, ascites.

Technical Specifications:

• Ligand: Polymyxin B
• Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 µm
• Matrix activation: Cyanogen bromide
• Attachment: Amino group
• Binding capacity: 200 – 500 µg LPS from E. coli serotype 0128:B12
• pH stability: 3 – 10
• Supplied as slurry in 50% glycerol containing 0.02% sodium azide

Additional Information

Additional Information