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Extensive studies have shown that individual Rock inhibitor (Thiazovivin), TGFβ inhibitor (SB431542), MEK inhibitor (PD0325901), GSK3β inhibitor (CHIR99021), , and their combinations (TSPC) can significantly enhance reprogramming efficiency of human somatic cells to induced pluripotent stem cells (iPSCs). For customers’ convenience, XcessBio has formulated the ready-to-use iPSC-TSPC cocktail containing all four small molecules at optimal concentrations and functionally tested it in iPSC reprogramming.

How to Use:

• In vitro: Dilute the received ready-to-use iPSC-TSPC cocktail at 1:1000 into your iPSC reprogramming medium to make the iPSC-TSPC media for iPSC induction.

Reference:

1. 1.  Valamehr B, et al.  A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. (2012) SCIENTIFIC REPORTS. 2 : 213
2. 2.  Li W, et al. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. (2009) Stem Cells. 27(12):2992-3000.
3. 3.  Lin T, et al. A chemical platform for improve induction of human iPS cells. (2009) Nature Method 6, 805-808.
4. 4.  Kawamata M, et al. Generation of genetically modified rats from embryonic stem cells. (2010) PNAS 107, 14223-14228.
5. 5.  Zhu S, et al. Reprogramming of human primary somatic cells by OCT4 and chemical compounds.(2010)  Cell Stem Cell 7, 651-655.
6. 6.  Yu J, et al. Efficient feeder-free episomal reprogramming with small molecules. (2011) PLoS ONE 6(3): e17557.

Products are for research use only. Not for human use.