Macrophages are cells differentiated from circulating bone
marrow-derived monocytes. In the bone marrow and subsequestly in the
blood and tissues, monocytes undergo a cascade of maturation that
culminate in morphologically and functionally mature tissue macrophages
[1]. Macrophages function to removal cellular debris and invading
pathogens. They phagocytize invading microorganisms, and scavenge dead,
damaged cells and cellular debris. Macrophages can be identified by the
specific expression of several cell surface proteins including CD14,
CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow
cytometry or immunohistochemical staining [2].
RMa-bm from ScienCell Research Laboratories are isolated from *****
CD® IGS rat bone marrow. RMa-bm are harvested at P0 and delivered
frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RMa-bm are
characterized by immunofluorescence with antibody specific to F4/80.
RMa-bm are negative for mycoplasma, bacteria, yeast, and fungi. RMa-bm
are guaranteed to further culture under the conditions provided by
ScienCell Research Laboratories; however, RMa-bm are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. #1921) for the culturing of RMa-bm in vitro.
