人角膜是一个独特的组织，因其透明且无血管。角膜包含三个不同的细胞层，外层上皮细胞、中间层基质和内层内皮细胞。角膜上皮细胞在先天免疫反应中起着作用，通过感应病原体存在并发出信号从而激活角膜防御系统。角膜上皮细胞还能高水平的表达乙醛脱氢酶，防止紫外线和4-羟基壬烯醛对细胞造成的伤害。角膜内皮细胞的更新由细胞因子调控，比如表皮生长因子，它能激活上皮细胞深层的同源受体。体外培养角膜内皮细胞已经可以替代毒理学检测中的动物实验。它们也可以被用来研究角膜细胞的增殖、分化和死亡之间的高度集成和平衡。
Sciencell研究实验室的HCEpiC是从人角膜分离得到的，原代冻存，冰冻运输。每管细胞密度*过5×10^5/ml。细胞特性经无血清培养基中镜检为鹅卵石形态，CK-18,和-19免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞10倍增增殖。
推荐培养基：角膜上皮细胞培养基 (CEpiCM, Cat. #6511)
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货号
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6510
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产地
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美国
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缩写
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HCEpiC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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2.) Vellonen KS, Mannermaa E, Turner H, H?kli M, Wolosin JM, Tervo T, Honkakoski P, Urtti A. (2010) "Effluxing ABC transporters in human corneal epithelium." J Pharm Sci. 99: 1087-98.
3.) Belser JA, Wadford DA, Xu J, Katz JM, Tumpey TM. (2009) "Ocular infection of mice with influenza A (H7) viruses: a site of primary replication and spread to the respiratory tract." J Virol. 283: 7075-84.
4.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
5.) Reichl S. (2008) "Cell culture models of the human cornea - a comparative evalsuation of their usefulness to determine ocular drug absorption in-vitro." J Pharm Pharmacol. 60: 299-307.
6.) Zhang JZ, Cavet ME, Ward KW. (2008) "Anti-inflammatory effects of besifloxacin, a novel fluoroquinolone, in primary human corneal epithelial cells." Curr Eye Res. 33: 923-32.
7.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
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