内皮细胞有很多生理功能，而且是许多病理进程的中心。肝脏包括两种内皮细胞：血管内皮细胞和肝窦内皮细胞。肝窦内皮细胞是微血管内皮细胞有独特的表型如同树突和如同CD4+T细胞的独特的抗原呈递细胞的功能。因此SEC表现为一种新的组织存在形式，非专业的抗原呈递细胞，其表现出与控制肝脏局部的免疫应答和免疫感应耐受性有关[1]。肝的微环境包括门静脉成分和血窦细胞增殖而来的可溶性介质，通过SEC严格控制抗原呈递来避免由免疫介导的损伤。SEC表达不同于大血管内皮细胞的特异性表面受体、有不同的形态和代谢[3]。研究表明SEC能够动态调节细胞表面裂孔隙度，在肝脏再生时对局部环境的区域性刺激能够快速的反应[2]。SEC功能紊乱和结构改变对整个肝脏有深远的影响。
Sciencell研究实验室的脐静脉内皮细胞是从人肝脏分离得到的，原代冻存，冰冻运输。每管细胞密度*过5×10^6/ml。细胞特性经白蛋白、cytokeratin-18 and vimentin免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。HH不能扩大培养或者产期培养，因为它不能增殖。
推荐培养基：内皮细胞培养基 (ECM, Cat. #1001)
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货号
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5000
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产地
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美国
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缩写
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HHSEC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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1.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15
2.) Lee SJ, Park SY, Jung MY, Bae SM, Kim IS. (2011) "Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver." Blood. 117: 5215-23.
3.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2010) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15.
4.) Guilini C, Urayama K, Turkeri G, Dedeoglu DB, Kurose H, Messaddeq N, Nebigil CG. (2010) "Divergent roles of prokineticin receptors in the endothelial cells: angiogenesis and fenestration." Am J Physiol Heart Circ Physiol. 298: H844-52.
5.) Huebert RC, Vasdev MM, Shergill U, Das A, Huang BQ, Charlton MR, LaRusso NF, Shah VH. (2010) "Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis." Hepatology. 52: 238-48.
6.) Kim Y, Larkin AL, Davis RM, Rajagopalan P. (2010) "The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers." Tissue Eng Part A. 16: 2731-41.
7.) Tang L, Yang J, Liu W, Tang X, Chen J, Zhao D, Wang M, Xu F, Lu Y, Liu B, Sun Q, Zhang L, He F. (2009) "Liver sinusoidal endothelial cell lectin, LSECtin, negatively regulates hepatic T-cell immune response." Gastroenterology. 137: 1498-508.e1-5.
8.) Jung MY, Park SY, Kim IS. (2007) "Stabilin-2 is involved in lymphocyte adhesion to the hepatic sinusoidal endothelium via the interaction with alphaMbeta2 integrin." J Leukoc Biol. 82: 1156-65.
9.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.
10.) Nahmias Y, Odde DJ. (2006) "Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures." Nat Protoc. 1: 2288-96.
11.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation." Am J Physiol Gastrointest Liver Physiol. 290: G535-42.
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