Human Apelin 12 (AP12) CLIA Kit EKU08645|产品详情|进口橙子视频旧款采购网

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Human Apelin 12 (AP12) CLIA Kit

品牌：

货号：EKU08645

规格：96T

货期：

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Human Apelin 12 (AP12) CLIA Kit

商品详情参考文献相关资料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	62.50-16000 pg/mL
Sensitivity	22.8 pg/mL
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time	2 hours
Gene Name	Apelin 12
Gene Aliases	APLN12
Application	CLIA
Assay Type	Competitive
Detection Method	Chemiluminescent
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Apelin 12 (AP12). No significant cross-reactivity or interference between Apelin 12 (AP12) and analogues was observed.
Test Principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Apelin 12 (AP12). A competitive inhibition reaction is launched between biotin labeled Apelin 12 (AP12) and unlabeled Apelin 12 (AP12) (Standards or samples) with the pre-coated antibody specific to Apelin 12 (AP12). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 12 (AP12) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Apelin 12 (AP12) level in the sample or standard.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 12 (AP12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ape
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism;