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Anti-NGAL (mouse) ABS 043-10 ABS 043-10|产品详情|进口橙子视频旧款采购网




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    Anti-NGAL (mouse) ABS 043-10
    品牌：
    货号：ABS 043-10
    规格：200 µg
    货期：

    Anti-NGAL (mouse)
    商品详情 参考文献 相关资料
    Cat. No.
    ABS 043-10
    Product name
    Anti-NGAL (mouse)
    Description

    Rat monoclonal antibody

    Specificity

    ABS 043-10 binds NGAL (neutrophil gelatinase-associated lipocalin) in mouse serum.

    Immunogen

    Recombinant mouse NGAL 

    Application
    ELISA, WB
    Species reactivity (positive)
    Mouse
    Species reactivity (negative)
    Human, pig, dog, rat
    Subclass

    IgG1/k

    Gene ID
    16819
    Clone number
    10
    Epitope

    Not determined

    Content

    Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

    Purification
    Protein-A purified
    Form
    Liquid
    Solvent
    0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
    Storage
    4-8ºC without exposure to light. No precautions necessary during handling.
    Target

    Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated in the liver. but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland.

    Application 1
    ELISA
    ABS 43-10 binds free mouse NGAL in solution. 
    ABS 043-10 forms an antibody pair for sandwich ELISA with ABS 043-29. It is recommended to use ABS 043-10 as capture antibody and biotinylated ABS 043-29 as detection antibody.
     
    The figure illustrates the calibration curve of a sandwich assay with ABS 043-10 as the capture antibody and ABS 043-29B as the biotinylated detection antibody.
    Application 2

    WB

    ABS 043-10 can be used in Western blotting

    1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

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