Description
The ViPrimePLUS Cryptococcus neoformans qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Cryptococcus neoformans genomes in clinical samples (e.g. cerebrospinal fluids, organ tissue and respiratory fluids).
Cryptococcus neoformans is a yeast-like fungus that causes Cyptococcosis, a pulmonary infection leading to pneumonia, meningitis, meningoencephalitis and other disseminated diseases, particularly in immunocompromised patients. In healthy individuals, colonization of the fungi in the respiratory airway can be harmless due to the ability of the immune defense to rid the progression of the infection. The fungi can be found in soil, thus inhalation of the spore into the lung allows primary infection to take place. Dissemination of the infection to the central nervous system and other parts of the body is not uncommon. Treatment of the pulmonary infection is via the administration of anti-fungal medicine.
ViPrimePLUS Cryptococcus neoformans qPCR Kit is intended for research use only.
Kit Component
-
C. neoformans Primers and Probe Mix (C. neoformans PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
DNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
Clinical samples (e.g. cerebrospinal fluids, organ tissue, respiratory fluids)
|
|
Gene Target
|
UDP-glucose epimerase (UGE1) gene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
• Internal Extraction Control (IEC) as inhibition control
• Positive control
|
|
Channels
|
• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM2021
|
ViPrimePLUS Cryptococcus neoformans qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Cryptococcus neoformans qPCR Kit
