Description
The ViPrimePLUS Streptococcus pyogenes qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Streptococcus pyogenes genomes in clinical samples (e.g. sputum and nasopharyngeal secretions).
Streptococcus pyogenes is a beta-hemolytic bacterium that belongs to group A streptococci (GAS). The pathogen causes a wide variety of respiratory tract, skin and invasive systemic infections. The infection usually begins with symptoms like sore throat and mild skin irritation such as impetigo. Invasive strains may spread to the lower skin epithelial layers, resulting in cellulitis and necrotizing fasciitis, which in turn will lead to amputation. Some S. pyogenes strains also release toxins such as erythrogenic toxin, streptolysin O and streptolysin S which cause scarlet fever and the streptococcal toxic shock syndrome (TSS). Scarlet fever is characterized by fever, a 'strawberry tongue' and a fine sandpaper rash over the upper body. TSS may result in multiorgan failure if immediate treatment is not sought after. Treatment is usually with a course of penicillin.
ViPrimePLUS Streptococcus pyogenes qPCR Kit is intended for research use only.
Kit Component
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S. pyogenes Primers and Probe Mix (S. pyogenes PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. sputum and nasopharyngeal secretions)
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Gene Target
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Collagen-like surface protein (sc1A) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2063
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ViPrimePLUS Streptococcus pyogenes qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Streptococcus pyogenes qPCR Kit
