Description
The ViPrimePLUS Simkania negevensis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Simkania negevensis genomes in human clinical samples.
Simkania negevensis is an obligate intracellular Gram-negative bacterium that belongs to the phylum Chlamydiae. It has been shown to be capable of replicating in amoeba and surviving in amoebal cycsts in drinking and reclaimed waste water. Although contact with infected secretions or person to person aerosol transmission is possible, it has been shown that domestic water supplies are also a possible source of infection.
ViPrimePLUS Simkania negevensis qPCR Kit is intended for research use only.
Kit Component
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S. negevensis Primers and Probe Mix (S. negevensis PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Human clinical samples
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Gene Target
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16S ribosomal RNA (16s rRNA)
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2054
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ViPrimePLUS Simkania negevensis qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Simkania negevensis qPCR Kit
