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Concentration
20- 50u/μl 
5'…CC↓SGG…3' 
3'…GGS↑CC…5'

Reaction Conditions 
1X Buffer V5 
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with BpuMI, less than 10% of the DNA fragments can be ligated.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of BpuMI for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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BpuMI {Cau II}