Concentration
5-30u/μl
5'…GCCNNNN↓NGGC…3'
3'…CGGN↑NNNNCCG…5'
Reaction Conditions
1X Buffer BgII
20mM Tris-HCl (pH 8.5), 10mM MgCl2, 200mM NaCl, and 1mM DTT. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with Bgl I, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Bgl I for 16 hours at 37°C.
Supplied with 10X Buffer Bgl I, 10X Buffer UB and Viva Buffer A. (Diluent)
*High enzyme concentration may result in Star Activity
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RV1142
|
Bgl I
|
600u
|
Downloads
Manual
Bgl I
