Concentration
10u/μl
5'…ACN↓GT…3'
3'…TG↑NCA…5
Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 65°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 5-fold overdigestion with Bst4CI, less than 10% of the DNA fragments can be ligated.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bst4CI for 16 hours at 65°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1204
|
Bst4CI {Tsp4CI}
|
150u
|
Downloads
Manual
Bst4CI {Tsp4CI}
Publication
This Product Has Been Used In:
Hamidizadeh, L., Abadi, R.H.H.B., Baigi, M.A.B., Dastsooz, H., Nejihad, A.K., Fardaei, M. (2015) Impact of KIF6 Polymorphism rs20455 on Coronary Heart Disease Risk and Effectiveness of Statin Therapy in 100 Patients from Southern Iran, Archives of Iranian Medicine, Vol. 18, No. 10 (2015).
