Concentration
10-40u/μl
5'…C↓CGG…3'
3'…GGC↑C…5'
Reaction Conditions
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 50mM NaCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with MspI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of MspI for 16 hours at 37°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
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Catalog No
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Description
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Pack Size
|
|
RE1302
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MspI
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1000u
|
Downloads
Manual
MspI
Publication
This Product Has Been Used In:
Mubarak, R., Haddad, S., Hamdan, O. (2016). Frequency of CYP1A1*2A Polymorphism in Syrian Children with Acute Lymphoblastic Leukemia, Research Journal of Pharmacy and Technology, Vol. 9, No. 2 (2016).
Nazree, N.E., et al. (2013) ) Lack of Association Between TPH2 Gene Polymorphisms with Major Depressive Disorder in Multiethnic Malaysia Population Asia-Pacific Psychiatryp.1-6.
