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Description
Chromo Taq DNA Polymerase is a thermostable DNA polymerase. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymerase catalyzed the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of MgCl₂ and has the 5' to 3' exonuclease activity. The enzyme is supplemented with inert color tracer dyes.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 20kb.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.
• Generates mostly 3' dA overhang amplification products which are suitable for TA cloning.

Unit Definition 
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ ml BSA in a final volume of 50μl.

Supplied With

• 10X ViBuffer A (without MgCl₂) 
  500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP
• 10X ViBuffer S
  160mM (NH₄)₂SO₄, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl₂ and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
• 50mM MgCl₂

Quality Control 
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.

Ordering Information

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Manual

Chromo Taq DNA Polymerase