Description
The ViPrimePLUS Botrytis cinerea qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Botrytis cinerea genomes in plant tissue samples.
Botrytis cinerea is an airborne plant fungi pathogen with a necrotrophic lifestyle attacking over 230 crop hosts worldwide. It causes huge economic losses in terms of yield and quality. Wet and cool weathers are the optimum conditions for fungal growth. A variety of defense mechanisms maintain the pathogen in a latent state in immature fruits, but these mechanisms diminish during berry ripening. Such fungal infection causes gray mold, also known as rotting in ripe fruits, especially after a long period of rainfall, close to harvesting. As the fungus is ever present in the environment, no preventive measures can totally rid of the organism. However, the spread of the disease can be reduced by removing dead leaves and infected fruits. After harvest, the fungus survives in the soil as small, black, inactive sclerotia on tilled-in leaves and fruit. Fungicides are commonly used to control the disease; however the traces of the residues in vegetable crops and soil may have harmful effect on human.
ViPrimePLUS Botrytis cinerea qPCR Kit is intended for research use only.
Kit Component
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B. cinerea Primers and Probe Mix (B. cinerea PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Plant samples
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Gene Target
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Species-specific fragment in the intergenic spacer region
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM3004
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ViPrimePLUS Botrytis cinerea qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Botrytis cinerea qPCR Kit
