Description
The ViPrimePLUS Infectious Hematopoietic Necrosis Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Infectious Hematopoietic Necrosis Virus genome in salmon fish samples.
Infectious Hematopoietic Necrosis Virus (IHNV) is a RNA virus which causes chronic disease in salmon and trout. IHNV is primarily found in the Pacific coast of the USA, Canada and Japan with different clinical isolates being responsible for disease in each area. Fish importation activity has introduced the disease to Europe and Asia, too. The virus is shed in urine, sexual fluids and external mucous and it can survive in the water for at least one month, thus the water-borne transmission. Vertical transmission has also been recorded. Once IHNV enters the host via the digestive tract and gills, it then attacks the internal organs of the host, especially spleen and kidney, causing necrosis of the tissue. Mortality mainly occurs due to edema and haemorrhage as the osmotic balance is impaired. Among the preventive steps taken to control the spread of the diseases include farm sanitation and disinfection of eggs. Raising the water temperature may limit the spread as well. Further research is needed to develop a more effective vaccine.
ViPrimePLUS Infectious Hematopoietic Necrosis Virus RT-qPCR Kit is intended for research use only.
Kit Component
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IHNV Primers and Probe Mix (IHNV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Fish samples
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Gene Target
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Matrix protein gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV4003
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ViPrimePLUS Infectious Hematopoietic Necrosis Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Infectious Hematopoietic Necrosis Virus RT-qPCR Kit
