Storage
Store at -20°C
Grade
Ultra Pure Grade
Description
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N’-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Specification
|
Appearance
|
White powder
|
|
Mol. Formula
|
C4H10O2S2
|
|
Mol. Weight
|
154.25
|
|
CAS RN
|
3483-12-3
|
|
Assay
|
Specifications
|
|
Titration ex –SH (iodometric)
|
≥99.0%
|
|
T.L.C.
|
One spot
|
|
UV Assay
|
|
|
1% Solution A280
|
<0.067
|
|
A305
|
<0.050
|
|
0.02M Solution A283
|
<0.040
|
|
1M Neutral Solution A400
|
<0.062
|
|
A405
|
<0.062
|
|
OD 700-500nm
|
≤0.035
|
|
Loss on Drying
|
<0.5%
|
|
Melting Point
|
42±2°C
|
|
Oxidized DTT
|
<0.5%
|
|
Solubility (10% Water)
|
Clear and colorless
|
|
I.R.
|
Consistent with assigned structure
|
|
DNase
|
None
|
|
RNase
|
None
|
|
Protease
|
None
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
PC0705 - 5g
|
DL-Dithiothreitol (DTT)
|
5g
|
|
PC0705 - 25g
|
DL-Dithiothreitol (DTT)
|
25g
|
|
PC0705 - 100g
|
DL-Dithiothreitol (DTT)
|
100g
|
Download
MSDS
DL-Dithiothreitol (DTT)
