Description
The ViPrimePLUS Neospora caninum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Neospora caninum genome in clinical faeces samples.
Neospora caninum is a protozoan parasite that primarily infects dogs and cattle causing neosporosis. It is also associated with sheep, goat, deer and horses. The transmission cycle between dogs and cattle occur when dogs pass out oocysts in their faeces, which are then ingested by the grazing cattle. Vertical transmission is observed in dogs and sheep which leads to abortion, premature birth and birth of animals with persistent infection. In cattle, N. caninum is an important cause of spontaneous abortion, infertility and reduction in milk yield. Dogs may develop rigid hyperextension, forelimb ataxia, head tremors, myocarditis and pneumonia, where the puppies will show neurological signs.
ViPrimePLUS Neospora caninum qPCR Kit is intended for research use only.
Kit Component
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N. caninum Primers and Probe Mix (N. caninum PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Ruminant samples (e.g. faeces)
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Gene Target
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Nc5 marker genomic sequence
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV6017
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ViPrimePLUS Neospora caninum qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Neospora caninum qPCR Kit
