Description
The ViPrimePLUS Plasmodium knowlesi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Plasmodium knowlesi genomes in clinical samples (e.g. blood).
Plasmodium spp. is an obligate protozoan that causes malaria, a common traveler's disease. There are about 200 species identified, and 5 of them infect human, i.e. P. falciparum, P. malariae, P. ovale, P. knowlesi and P. vivax. These parasites exploited mosquitoes of the Aedes, Culex, Mansonia, Theobaldia and Anopheles genera to transmit them to different vertebrate host via biting. Symptoms of the infection includes fever, chills, fatigue, headache, nausea, anemia, diarrhea and in severe cases, pulmonary and renal dysfunction. Effective treatment is given based on the parasites' species identification. Now, the gold standard of the diagnosis is microscopic examination of blood smears, which lacks of sensitivity and requires considerable expertise. Therefore, real time PCR offers a fast, sensitive and specific solution to the diagnosis.
ViPrimePLUS Plasmodium knowlesi qPCR Kit is intended for research use only.
Kit Component
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P. knowlesi Primers and Probe Mix (P. knowlesi PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. blood)
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Gene Target
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Plasmepsin gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM4012
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ViPrimePLUS Plasmodium knowlesi qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Plasmodium knowlesi qPCR Kit
