Description
The ViPrimePLUS Gallid herpesvirus 1 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Gallid herpesvirus 1 genome in tracheal samples, tissue and infected eggs.
Gallid herpesvirus 1 (GaHV-1) is a double-stranded DNA virus that infects chickens and many other birds. It is therefore also known as avian herpesvirus 1. The infection causes respiratory disease known as avian infectious laryngotracheitis (ILT). The clinical signs vary from very severe which leads to birds’ death due to asphyxiation, to relatively mild respiratory symptoms. Infected birds can be the long-term silent carrier of the latent virus, where the virus will be re-excreted at a later time without symptoms. GaHV-1 is shed in respiratory secretions and transmitted via aerosol inhalation or direct contact with contaminated objects. Egg production yield and quality will decrease due to the respiratory distress. Morbidity and mortality rate of the infection may exceed 50%. Currently, the available vaccines are in live attenuated form but further research on using genetically engineered vaccines is under progress.
ViPrimePLUS Gallid herpesvirus 1 qPCR Kit is intended for research use only.
Kit Component
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GaHV-1 Primers and Probe Mix (GaHV-1 PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Avian samples (tracheal samples, tissue, infected eggs)
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Gene Target
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Transcriptional regulator (ICP4) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV1016
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ViPrimePLUS Gallid herpesvirus 1 qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Gallid herpesvirus 1 qPCR Kit
