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产品介绍
品名	Polyclonal Antiserum to Human C9 Protein
货号	A310
规格	2.0 mL
标本类型	Unformed stool
防腐剂	≤ 0.1% Sodium Azide
物种交叉反应	Baboon, Horse, Rabbit, Hamster, Rhesus macaque
储存	2°C to 8°C  (≤ 30 days) For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.
使用说明	For Research Use Only. Not for use in diagnostic procedures
产品描述	Highly purified human C9 was isolated from normal serum and used to immunize goats. The anti-human C9 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C9 at varying concentrations
背景	C9 is a glycoprotein which is present in normal human serum/plasma at approximately 55 µg/mL. Primarily synthesized in the liver, C9 is a single-chain protein with a molecular weight of approximately 71 kD. This complement protein is amphiphilic in nature, and can be fragmented by human α-thrombin to produce C9a and C9b. The hydrophilic domain of the C9 complex becomes the C9a fragment and has an approximate molecular weight of 34 kD. The hydrophobic end becomes C9b fragment, which has an approximate molecular weight of 37 kD.
应用	Applications of the C9 polyclonal antisera have been evalsuated by various research facilities, and include Western Blot, 1 Immunodepletion, 2 Immunofluorescence, 3,4,5 IHC, 6,7 and ELISA. 8
参考文献	Marks, R., Hindmarsh, E. Complement activation occurs on subendothelial extracelluslar matrix in vitro and is initiated by retraction or removal of overlying endothelial cells, J Immunol 160:6128-6136, 1998. Barbashov, S., Wang C., Nicholson-Weller, A. Serum amyloid P component forms a stable complex with human C5b-6, J. Immunol. 158:3830-3835, 1997