Feature

Ethidium Bromide

SYBR Green I

Fluorescence

Red (615 nm)

Green (524 nm)

Excitation maximum

302 nm

454 nm

Excitation light source

UV only

Blue light or UV

Sensitivity

2 ng / band(dsDNA)
100 ng / band (RNA)

0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)

Health hazard

High

Low

Appearance:

orange solution

Quality control:

NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

MSDS:

Download

Excitation maximum, nm:

454

Extinction coefficient at excitation maximum, Lmol-1cm-1:

73000

Emission maximum, nm:

524

Fluorescence quantum yield:

0.8